Mazomanie is a village in Dane County, Wisconsin, United States. The population was 1,652 at the 2010 census. The village is located within the Town of Mazomanie. It is allowance of the Madison Metropolitan Statistical Area.

Mazomanie is located at 43°10′28″N 89°47′41″W / 43.17444°N 89.79472°W / 43.17444; -89.79472.

According to the United States Census Bureau, the village has a total area of 1.86 square miles (4.82 km), of which, 1.83 square miles (4.74 km²) of it is estate and 0.03 square miles (0.08 km) is water.

The land upon which rests the town of Mazomanie fell within the hunting grounds of the Hočąk, or Winnebago, Indian nation. About a decade after the Winnebago cession of 1832, there were unaided a small number of white settlers in the area. What precipitated the birth of the town was the advent of the Milwaukee and Mississippi Railroad in 1855, which passed through the region to attach Milwaukee similar to La Crosse. The bureaucrat of the railroad, Edward Brodhead, gave the village its name; many years later, he explained its derivation: “He (Mazomanie) was an Indian chief in our let in and was competently known to the antiquated gentleman, H.L. Dousman, who said the Indians pronounced it as even if it was spelled Man-zo-ma-nie and the English of it is Iron Horse, which I adopted for the herald of a railroad town and as well as for the pronounce of my horse.” This distinguished chief was Mą́zamąnį́ga, “Iron Walker.”