Duboistown is a borough in Lycoming County, Pennsylvania, United States. The population was 1,198 at the 2020 census. It is allowance of the Williamsport, Pennsylvania Metropolitan Statistical Area.

Duboistown (pronounced ‘doo-BOYS town’) is named for its founders John and Mathias DuBois who bought 489 acres (1.98 km) of land between 1852 and 1857. The DuBois brothers separated their estate into parcels and customary the village that bears their name. John DuBois left the West Branch Susquehanna Valley back Duboistown was expected as a borough. He sold his matter interests and moved west to Clearfield County. He became quite rich and the city of DuBois was named in his honor.

The town built by the DuBois brothers and traditional as a borough in 1878 is by no means the coming on of the chronicles of Duboistown. It is situated at the mouth of Mosquito Run on the banks of the West Branch Susquehanna River. A tribe of Susquehannock Indians had what appears to have been a fairly major treaty at the mouth of the creek. The forward European settlers found the remains of an Indian village there. Arhaeologic evidence of earthenware, soapstone ware, pestles, hatchets, ornaments and charms were found upon the house that is across the river from Lycoming Creek and close where the Sheshequin Path crossed the river.

The land on which Duboistown is located was first surveyed in 1769. At the get older it was known as “Walnut Bottom” for the immense stands of black walnut that covered the alluvial plain on which the borough now stands. Samuel Boone, cousin of Daniel Boone, held the first warrant for home at Walnut Bottom.