Jamestown is a home rule-class city in Russell County, Kentucky, in the United States. It is the chair of its county. The population was 1,794 at the 2010 U.S. census.

Jamestown is located at 36°59′5″N 85°4′1″W / 36.98472°N 85.06694°W / 36.98472; -85.06694 (36.984730, -85.066840). According to the United States Census Bureau, the city has a total Place of 2.2 square miles (5.7 km²), all land.

The community was established in 1826 to be the chair of the newly formed Russell County. It was briefly known as Jacksonville in honor of General Andrew Jackson, who had just won a plurality of the popular vote during the 1824 presidential election but directionless the runoff in the House of Representatives, largely because of the “corrupt bargain” struck by Henry Clay and John Quincy Adams. However, an anti-Jacksonian party loyal to Clay came into capacity the same year and the make known office was customary in November as Jamestown after local landowner James Wooldridge, who (together subsequent to his brother John) had donated 110 acres of house for the town. The war of the state legislature incorporating the city in December continued this name, which the city has used since.

At the 2000 census there were 1,624 people in 662 households, including 421 families, in the city. The population density was 722.5 inhabitants per square mile (279.0/km). There were 755 housing units at an average density of 335.9 per square mile (129.7/km²). The racial makeup of the city was 94.89% White, 3.51% African American, 0.43% Asian, 0.12% from extra races, and 1.05% from two or more races. Hispanic or Latino of any race were 0.86%.