Hustonville is a house rule-class city in Lincoln County, Kentucky, in the United States. The population was 405 at the 2010 census. It is ration of the Danville Micropolitan Statistical Area.

The community was originally known as The Crossroads from its location upon trails connecting the Kentucky and Green rivers and the Falls of the Ohio as soon as Logan’s Fort (present-day Stanford). It was after that known as Farmington and, after the 1818 erection of a proclaim office, Hanging Fork after a local stream named for two bandits who were hanged by Virginia officers rather than escorted incite for trial. For three months in 1826, it was known as New Store, but the name subsequently returned to Hanging Fork. When the town was established upon February 29, 1836, it was renamed Hustonville after two local landowners; the broadcast office adopted the name the adjacent year. The city was formally incorporated by the let in assembly in 1850.

During the mid 19th and forward 20th centuries, Hustonville was home to Christian College. The moot was received following a fundraising demonstrate by William Logan Williams, who had been instrumental in securing child maintenance for church construction in the city. Future U.S. senator and Governor of Kentucky, Augustus O. Stanley, served as chair of belles-lettres at the college in 1890.

According to the United States Census Bureau, the city has a total area of 0.6 square miles (1.6 km), all land. The city is concentrated along Kentucky Route 78 (Main Street), just east of its intersection past U.S. Route 127, in northwestern Lincoln County. The city lies close the headwaters of Hanging Fork Creek, a tributary of the Dix River.