Glencoe is a home rule-class city in Gallatin County, Kentucky, in the United States. The population was 360 as of the 2010 census, up from 251 at the 2000 census.

A publish office was established in the community in 1848 and named for Glen Coe in Scotland, the site of the Massacre of Glencoe. It was formally incorporated by the welcome assembly in 1960.

Glencoe is located in southeastern Gallatin County at 38°42′48″N 84°49′19″W / 38.71333°N 84.82194°W / 38.71333; -84.82194 (38.713207, -84.821972), in the valley of Eagle Creek. The creek forms allowance of the southern boundary of the city and along with is the Owen County line. U.S. Route 127 passes through the city, leading south 43 miles (69 km) to Frankfort, the permit capital. Kentucky Route 467 crosses US 127 in the center of Glencoe, leading east 15 miles (24 km) to Dry Ridge and west 5 miles (8 km) to Sparta. The Glencoe city limits extend north from the center of town 2 miles (3 km) along US 127 to Exit 62 upon Interstate 71. From this point, I-71 leads northeast 33 miles (53 km) to Covington and southwest 64 miles (103 km) to Louisville.

According to the United States Census Bureau, the city has a total area of 1.8 square miles (4.6 km), of which 0.01 square miles (0.03 km²), or 0.65%, is water. Eagle Creek, which forms the southern edge of town, is a west-flowing tributary of the Kentucky River and ration of the Ohio River watershed.