Worthington Springs is a spring and town in Union County, Florida, United States. The population was 181 at the 2010 census. According to the U.S. Census Bureau’s 2018 estimates, the town had a population of 380.

The community was named for to the fore settler Sam Worthington, who arrived prior to the first Seminole War of 1814–1819. The mineral spring on the Santa Fe River was alleged to have medicinal benefits, attracting people from the late 1800s to the in the future 1900s. In the into the future years, swimming was segregated, with men and women assigned specific times intervals. In 1906, the extra owner constructed a 12’×12′ concrete bin around the spring to occupy water into a 90’×50′ concrete pool with a wall dividing the bathing Place into sections for men and women. The obscure included a hotel, recreation hall and bathhouse for the spring pool.

Independence Day was always a big celebration at the springs. Thousands of people from surrounding communities would converge upon the springs to picnic, swim, politic and converse with contacts and fellow Floridians. A special train transported people from Jacksonville for the holiday festivities.
An iron and timber bridge was built across the river in 1908, facilitating right of entry to and from the south. It was replaced in 1937 as soon as the gift concrete structure.
In the mid-twentieth century, the outflow from the spring declined, and later stopped flowing completely. Activity at the resort likewise curtailed, and the capability was abandoned. The area around the Santa Fe river is subject to periodic flooding, just as the flow at the spring varies widely. In 1972, flow was measured at 233,280 gallons per day.

Worthington Springs was incorporated in 1963 as soon as a Mayor–council government. Street lights were installed, and a playground was created in the town park. In 1975, a Community Center was built the included admin offices for a City Hall.