Palmyra is a village in Jefferson County, Wisconsin, United States, along the Scuppernong River. It was named after the desert oasis city of Palmyra, Syria, due to its dry, sandy soil. The population was 1,719 at the 2020 census. The village is adjacent to the Town of Palmyra.

The Town of Palmyra was established in 1846. In 1876, part of the Town of Palmyra was incorporated as the village of Palmyra.

Palmyra was known for its mineral springs. The Palmyra Springs Sanitarium was customary in 1870, situated on what is known today as Lower Spring Lake. The sanitarium was a substantially built four-story brick structure containing spacious halls, wide verandas, and futuristic health and wellness accessories. The building contained facilities for the administration of various forms of electro-shock massage. The rooms were expansive and luxuriously furnished, providing a variety of salt, shower, shampoo, natural mineral, Turkish, and Russian style baths. There was a large gymnasium, theater, music hall, billiard parlors, bowling alleys, archery and tennis courts. The surrounding forty-acre reforest of indigenous oaks was referred to as the Sanitarium Grove – today portion of the Southern Kettle Moraine State Forest – which contained winding pedestrian paths and resting nooks. The sanitarium was one and half miles from a large spring known by publish as the Aurelian Spring, the Great Geyser Spring, or the Big Blue Spring. It was 50 feet across, 38 feet deep, with a flow of Definite crystal-blue water that was believed to have therapeutic remedial value. A dam and spillway was well ahead built downstream of the Great Geyer Spring, forming what is today known as Blue Spring Lake. In 1924, the sanitarium became the Druggist’s National Home, a house for aged and infirm druggists, but a few years later, interest in the therapeutic mineral treatments declined and the power was closed. In the late 1950s, the building was raised and the house leveled.

Palmyra is located at 42°52′37″N 88°35′13″W / 42.87694°N 88.58694°W / 42.87694; -88.58694 (42.87721, -88.587117).