Tildenville is a census-designated place (CDP) and an unincorporated area in Orange County, Florida, United States. The population was 511 at the 2010 census. It is portion of the Orlando–Kissimmee Metropolitan Statistical Area. Generally speaking, Tildenville is a narrow Place alongside Orange County Road 545, south of Florida State Road 50 and north of the Stoneybrook West planned unit development. Residents utilize Winter Garden, Florida for mail delivery. The CDP takes its broadcast from Luther F. Tilden, a prominent speculator and citrus magnate who greatly contributed to the community. Historically, Tildenville referred to an Place larger than the unprejudiced CDP. Landmarks such as Tildenville Elementary School, Tildenville Marketplace, and Meadow Marsh are now located within the city limits of Winter Garden.

Tildenville is located at 28°32′22″N 81°36′2″W / 28.53944°N 81.60056°W / 28.53944; -81.60056 (28.539309, -81.600432).

According to the United States Census Bureau, the CDP has a total area of 1.1 km (0.4 mi²), of which 0.9 km (0.3 mi²) is house and 0.2 km (0.1 mi²) (14.63%) is water.

At the 2000 census, there were 513 people, 169 households and 129 families residing in the CDP. The population density was 565.9/km² (1,465.7/mi). There were 180 housing units at an average density of 198.6/km² (514.3/mi). The racial makeup of the CDP was 13.84% White, 80.51% African American, 3.51% from additional races and 2.14% from two or more races. Hispanic or Latino of any race were 11.89% of the population.