Masaryktown is an unincorporated community and census-designated place (CDP) in Hernando County, Florida, United States. The population was 1,077 at the 2020 census. It is named after the first president of Czechoslovakia, Tomáš Garrigue Masaryk.

Masaryktown is located along the southern edge of Hernando County at 28°26′30″N 82°27′38″W / 28.44167°N 82.46056°W / 28.44167; -82.46056 (28.441708, -82.460633). It is bordered to the north and west by Spring Hill, to the northeast by Garden Grove, and to the south in Pasco County by Shady Hills. U.S. Route 41 runs through the eastern side of the community, leading north 9 miles (14 km) to Brooksville, the Hernando County seat, and south 17 miles (27 km) to Land o’ Lakes. Tampa is 36 miles (58 km) to the south.

According to the United States Census Bureau, Masaryktown has a total area of 1.0 square mile (2.7 km), of which 0.004 square miles (0.01 km²), or 0.53%, are water.

Masaryktown was founded in 1924 and named after Tomáš Garrigue Masaryk, the founder and first president of Czechoslovakia. Of the original 61 persons who met to found the town, 60 were Slovak and on your own one was Czech.  They formed the Hernando Plantation Company and sold at a price of $1,000.00 per share, which settled the right for each share-owner to buy 20 acres of land. The settlers first planted oranges, but killer frosts devastated the orchards. Many Slovaks eventually left, and by 1952, only 15-25 families remained to carry upon a more prosperous enterprise in chicken farming. But even they declined by 1965 due to intense competition.